Estudia
- Artes y humanidades
-
Ciencias
- Máster Erasmus Mundus en Recursos Biológicos Marinos
- Máster Universitario en Análisis de Datos para la Inteligencia de Negocios
- Máster Universitario en Biotecnología Alimentaria
- Máster Universitario en Biotecnología Aplicada a la Conservación y Gestión Sostenible de Recursos Vegetales
- Máster Universitario en Biotecnología del Medio Ambiente y la Salud
- Máster Universitario en Ciencias Analíticas y Bioanalíticas
- Máster Universitario en Conservación Marina
- Máster Universitario en Física Avanzada: Partículas, Astrofísica, Nanofísica y Materiales Cuánticos
- Máster Universitario en Modelización e Investigación Matemática, Estadística y Computación*
- Máster Universitario en Química Teórica y Modelización Computacional
- Máster Universitario en Química y Desarrollo Sostenible
- Máster Universitario en Recursos Geológicos e Ingeniería Geológica
- Ciencias de la salud
- Ciencias sociales y jurídicas
- Ingeniería y arquitectura
- Información, acceso y becas
Métodos de Análisis Basados en el Uso de Isótopos Estables
- Clases Expositivas (17.5 Hours)
- Tutorías Grupales (1 Hours)
- Prácticas de Aula/Semina (4 Hours)
This subject belongs to the MODULE 2 (optative, theoretical), which has 3 different subjects lasting all of them 3 ECTS. Specifically, this is one will be taught in English, as part of the final aim of converting the whole master into a full international degree in a short period of time.
This subject will cover the most important applications of the use of isotope ratios of the elements. The basis and the applications of isotope ratios will depend on the type of ionization source employed such as atomic (ICP-MS) or molecular (Electron Impact, electrospray or MALDI). Special attention will be paid to quantitative applications both in inorganic and organic analysis based on isotope dilution. As isotope dilution analysis is a primary method directly traceable to the international system of units, this subject is particularly related to the subject “Chemometrics and advance data treatment”.
It is worth stressing that the University of Oviedo is worldwide regarded as one of the reference institutions in the measurement of isotope ratios by Mass Spectrometry and in the development of analytical methodologies based on IDMS. For these reasons there are several teachers that are specifically trained on the different topics of the subject. Moreover, the Mass Spectrometry service of the University of Oviedo offers a wide range of mass spectrometers including Multicollector-ICP-MS (Neptune, Thermo), double focussing magnetic sector field ICP-MS (Element 2, Thermo), quadrupole ICP-MS (Agilent 7500), LC-MSMS (Agilent 6460), GC-MS and GC-MSMS (Agilent 7000) and isotope ratio mass spectrometers (LC-IRMS and GC-IRMS from Thermo). For more information visit the web page: http://www.sct.uniovi.es/index.php?option=com_content&task=view&id=66&Itemid=127
The requisites match up with the general ones of the master degree:
“The prerequisite for entry the master will be a first cycle degree in one of the following disciplines:
- Bachelor degree in chemistry
- Bachelor degree in the related disciplines: Physics, Geology, Biology, Biochemistry, Pharmacy, Medicine, Environmental Sciences and Food technology
- Bachelor degree in engineering chemistry
- Other related Bachelor degrees in Sciences and Life Sciences.
In the case of students from other countries which could have studied different disciplines, every specific case will be evaluated to check if the student profile matches the prerequisites of the master.”
The expected basic competences will be:
CB-6.- Ability to understand the knowledge required to be original in the development and application of ideas, usually in a research context
CB-8 Ability to integrate knowledge and handle complexity, and formulate judgements with incomplete or limited information, but that include reflecting on ethical responsibilities linked to the application of their knowledge and judgements
CB-9 Ability to communicate their conclusions, and the knowledge and rationale underpinning these, to specialist and non-specialist audiences clearly and unambiguously.
General Competences:
CG-1 Ability to analyse material and synthesise concepts
CG-4 Information-management competences, in relation to primary and secondary information sources and ability to manage adequately such obtained information.
Specific Competences:
CE-1 Knowledge of the basis for the main analysis techniques, both already established and state-of-the-art techniques, and the related experimental methodologies.
CE-2 Skills required for the conduct of advanced laboratory procedures and use of instrumentation in synthetic and analytical work both in a research and routine lab.
CE-3 Apply the chemometric concepts to experimental design and data treatment.
CE-5 Ability to choose the right analytical technique for the analysis and characterization of different materials and nanomaterials.
CE-6 Ability to choose the right analytical technique for the analysis, characterization and quantification of compounds of interest, both inorganics and organics, in complex samples.
CE-7 Ability to assimilate and evaluate laboratory results to solve problems efficiently
Student learning outcomes:
- RA-1: The student should demonstrate knowledge and understanding of essential facts, concepts, principles and theories relating to the subject areas studied during the Master program.
- RA-2: The student should be able to read and understand scientific papers published in international journals.
- RA-3: The student should be able to elaborate correctly a report on a specific research topic and defend it in front of an especilized audience
- RA-4: The student should be able to apply the knowledge acquired to resolve complex problems arised in the problem-solving classes.
The contents of the subject will be:
1. Introduction to the concept of isotope. Stable and radioactive isotopes. Natural variations of isotope abundances.
2. Measurement of isotope ratios of “light” elements and related applications.
3. Measurement of isotope ratios of “heavy” elements and related applications.
4. Basis of isotope dilution analysis. Isotope pattern deconvolution.
5. Elemental isotope dilution: analysis of metals.
6. Isotope dilution analysis for elemental speciation.
7. Molecular isotope dilution analysis: Determination of organic compounds.
8. Enriched stable isotopes as tracers in the study of metals, organometals and organic compounds environmental reactivity.
9. Enriched stable isotopes as tracers in the study of metabolism in plants and animals.
10. Enriched stable isotopes in quantitative proteomics.
The wide variety of learning and teaching approaches used are divided into 2 big groups:
On-site activities:
- 16 lectures (one hour each), supported by multimedia teaching techniques, where the basis of MS will be introduced to the students.
- 4 problem-solving classes (one hour each) where different topics and problems, previously worked out by the students, will be discussed and solved.
- 1 small group teaching.
Distance activities:
- Individual work: The student should devote time to assimilate the concepts and background introduced by the teacher during the lectures. In addition, the student should go into the main topics using primary and secondary information sources, including information retrieval through on-line computer searches. Problem-solving classes will be used to clarify questions raised during such work.
- Team work: The teaching in smaller groups will be performed in groups. Such groups will be maintained for any other activity which could take place, for example, in problem-solving classes. Also, ability to interact with other people and to engage in team-working will be promoted.
Student workload (hours) for each specific section is given in the following table:
On-site work | Distance work | |||||||||||
Sections | Hours | Lectures | Problem solving classes | Laboratory practices, | Clinical practices | Small-group teaching | External practices | Evaluation sessions | Total | Team-work | Individual work | Total |
Section 1 | 4,6 | 1 | 0,1 | 1,1 | 0,5 | 3 | 3,5 | |||||
Section 2 | 9,1 | 2 | 0,1 | 2,1 | 1,5 | 5,5 | 7 | |||||
Section 3 | 12,2 | 2 | 2 | 0,2 | 4,2 | 2,5 | 5,5 | 8 | ||||
Section 4 | 12,2 | 2 | 2 | 0,2 | 4,2 | 2,5 | 5,5 | 8 | ||||
Section 5 | 6,1 | 1 | 0 | 0,1 | 1,1 | 1,5 | 3,5 | 5 | ||||
Section 6 | 6,2 | 2 | 0 | 0,2 | 2,2 | 1 | 3 | 4 | ||||
Section 7 | 5,7 | 2 | 0,2 | 2,2 | 0,5 | 3 | 3,5 | |||||
Section 8 | 4,6 | 1 | 0,1 | 1,1 | 0,5 | 3 | 3,5 | |||||
Section 9 | 4,6 | 1 | 0,1 | 1,1 | 0,5 | 3 | 3,5 | |||||
Section 10 | 9,7 | 2 | 1 | 0,2 | 3,2 | 0,5 | 6 | 6,5 | ||||
Total | 75 | 16 | 4 | 0 | 1 | 1,5 | 22,5 | 11,5 | 41 | 52,5 |
Teaching and learning methods | Hours | % | Total | |
On-site | Lectures | 16 | 21,3 | 22,5 |
Problem-solving classes | 4 | 5,3 | ||
Laboratory practices | -- | -- | ||
Clinical practices | -- | -- | ||
Small-group teaching | 1 | 1,3 | ||
External practices | -- | -- | ||
Evaluation sessions | 1,5 | 2 | ||
Distance | Team work | 11,5 | 15,3 | 52,5 |
Individual work | 41 | 54,7 | ||
Total | 75 | 100 |
The procedures, different aspects and the criteria used for the assessment of student performance is given in the following table. The weight of each assessment procedure in the final mark of the student (in both examination sessions: regular and extra) is also given:
Procedure | Assessment criteria | Instrument | Weight (%) |
Activities in the teaching classes | Participation and interest shown during the problem-solving and small-group teaching classes. | Exercises. Seminars. Personal assessment of the teacher. | 20 % |
Written report on an specific topic | Command of the matter and degree of attainment of the expected learning outcomes. | Written report | 80 % |
General:
- Isotope Dilution Mass Spectrometry, J. Ignacio García Alonso and Pablo Rodriguez González, Royal Society of Chemistry, 2013.
- “Stable Isotope Geochemistry, Jochen Hoefs, Ed. Springer, 5ª edición, 2004
- Lectures in Isotope Geology, E. Jäger and J.C. Hunziker, Ed. Springer, 1979.
- Inductively Coupled Plasma Spectrometry and its Applications, S. Hill, Ed. Blackwell Publishing, 2ª edición, 2007.
- Análisis Químico de Trazas, Carmen Cámara y Concepción Pérez Conde (eds), Ed. Síntesis, Capítulo 4, La dilución isotópica en el análisis de trazas, J.I. García Alonso, P. Rodríguez González, 2011.
Specific
- Application of heavy stable isotopes in forensic isotope geochemistry: A review, J. Aggarwal, J. Habicht-Mauche, C. Juarez, Applied Geochemistry, 2008, 23, 2658-2666
- Application of enriched stable isotopes as tracers in biological systems: a critical review, S. Stürup, H.R. Hansen, B. Gammelgaard, Analytical and Bioanalytical Chemistry, 2008, 390, 541-554.
- The evolution and application of multicollector ICPMS, C.B. Douhitt, Analytical and Bioanalytical Chemistry, 2008, 390, 437-440.
- Isotope dilution analysis for elemental speciation: a tutorial Review, P. Rodríguez González, J.M. Marchante Gayón, J.I. García Alonso, A. Sanz-Medel, Spectrochimica Acta Part B, 2005, 60, 151-207.
- Applications of isotope dilution-mass spectrometry in clinical chemistry, pharmacokinetics, and toxicology, A.P. De Leenheer, L.M. Thienpont, Mass Spectrometry Reviews, 1992, 11, 249-307.
- Mass Spectrometry-based Proteomics turns Quantitative, S-E. Ong, M. Mann, Nature Chemical Biology, 2005, 1, 252-262.
- Stable Isotopic labelling in proteomics, K. Gevaert, F. Impens, B. Ghesquiere, P. Van damme, A. Lambrechts, J. Vandekerckhove, Proteomics, 2008, 8, 4873-4885